How it works
A spectrophotometer measures how much light a sample lets through. Two drawings: the path of the light from the lamp to the detector, then a benchtop instrument with its display, wavelength control and sample compartment.
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The path of the light
The lamp makes white light, and the lens gathers it onto the entrance slit. Inside the monochromator the diffraction grating spreads the light into a spectrum. The exit slit lets one narrow band out. That band passes through the cuvette and reaches the detector.
From light to a reading
The detector turns the light that reaches it into an electrical signal, and the display shows it. Turning the wavelength control moves the grating, so a different part of the spectrum lines up with the exit slit.
- A cuvette can be a small square tube, as drawn here, or a round test tube.
Questions and answers
What are the main parts of a spectrophotometer?
A lamp for the light, a monochromator with an entrance slit, a diffraction grating and an exit slit, a sample compartment that holds the cuvette, a detector, and a display.
What does the monochromator do in a spectrophotometer?
It turns white light into light of one narrow band. Its grating, or in some instruments a prism, spreads the light into a spectrum, and the exit slit lets only the chosen part through to the sample.
What is a cuvette?
A cuvette is the small clear container that holds the liquid sample in the beam. The light goes in one side and out the other on its way to the detector.
What does the detector do in a spectrophotometer?
It catches the light that comes through the sample and turns it into an electrical signal, which the instrument measures and shows on the display.