How it works
The electron transport chain sits in the inner membrane of a mitochondrion. The first chart follows the electrons from NADH and FADH₂ to oxygen; the second shows the hydrogen ions flowing back through ATP synthase.
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Follow the electrons, then the hydrogen ions
NADH hands its electrons to Complex I and FADH₂ hands its electrons to Complex II. Ubiquinone carries them to Complex III, cytochrome c carries them to Complex IV, and there oxygen takes them and becomes water. Complexes I, III and IV pump hydrogen ions out of the matrix as the electrons pass; Complex II does not. The ions build up in the intermembrane space, then flow back through ATP synthase, which joins ADP and phosphate into ATP. The chain and ATP synthase together are oxidative phosphorylation.
- The shaded side of the membrane is the intermembrane space; the matrix is below.
- Blocks with an arrow through them pump hydrogen ions.
Questions and answers
What are the parts of oxidative phosphorylation?
The electron transport chain and ATP synthase. The chain is Complexes I, II, III and IV with two carriers, ubiquinone and cytochrome c. It builds a hydrogen ion gradient across the inner membrane, and ATP synthase uses the gradient to make ATP.
What is the order of the electron transport chain?
From NADH: Complex I, ubiquinone, Complex III, cytochrome c, Complex IV, oxygen. From FADH₂ the electrons enter at Complex II and then follow the same path from ubiquinone on.
Where is the electron transport chain located?
In the mitochondrion's inner membrane. Hydrogen ions are pumped out of the matrix to the intermembrane space, and ATP is made on the matrix side.
What is the final electron acceptor?
Oxygen. At Complex IV it takes the electrons, picks up hydrogen ions and forms water.