How it works
Label the parts of a micropipette by number. 1 to 5 are on the handle, from the button on top down to the grip; 6 to 9 are on the narrow end below it, down to the tip.
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Micropipette word bank
In alphabetical order: body (grip), disposable tip, piston, plunger button, shaft, tip ejector (arm and collar), tip ejector button, volume display, volume knob.
Numbers that get swapped
Two buttons sit at the top: 1 is the one on the very top (plunger button), 2 the smaller one beside the ridged ring (tip ejector button). 2 is the button only: the arm it pushes, down the side of the narrow tube, is 8 (tip ejector). Two lines end on that tube: 7 on the rod seen through the cut-away (piston), 6 on the tube itself (shaft). 3 is the ridged ring (volume knob), 4 the small window in the handle (volume display).
- When marking, volume adjustment dial is right on line 3, and digital volume indicator or volume window on line 4.
- The thin stem under the plunger button and the liquid in the tip are drawn but carry no number.
Questions and answers
What is the difference between the plunger and the piston of a micropipette?
The plunger button is outside, on top, and is the part you press. The piston is inside the shaft: a metal or ceramic rod in an airtight sleeve, driven by the plunger. On the worksheet they are numbers 1 and 7.
Is the volume display on a micropipette digital?
It is often called the digital volume indicator, but digital here means that it shows digits. On a manual micropipette it is a dial display, and nothing in it is electronic.
What does the tip ejector do on a micropipette?
It takes the used tip off. Pressing the tip ejector button, number 2, moves the arm and collar, number 8, and the tip is cast off without being handled.
What are micropipette tips made of?
Plastic, in general polypropylene, and they are made to be used once. Most have a color code, and the pipette they fit carries the same color.
Why is it called an air displacement micropipette?
Because the piston moves air, not liquid. As the piston travels up it leaves a vacuum, air from the tip rises to fill it, and liquid is drawn up into the tip in its place.