How it works
This translation diagram labeled part by part shows a ribosome part way along an mRNA, reading it from 5' to 3', left to right. The simple set asks the 8 main parts; the full set adds the three sites and the codons that start and stop it.
Read the full guide
Reading the code
The mRNA runs between the small and large ribosomal subunits. Each codon, three bases, is matched by the anticodon of a tRNA carrying one amino acid. Reading began at the start codon, AUG, and will end at a stop codon.
A, P and E
A new tRNA enters the A site. A peptide bond joins its amino acid to the chain held by the tRNA in the P site. The ribosome then moves one codon along: the chain's tRNA shifts to the P site and the empty one leaves from the E site.
- Read the sites in the order a tRNA passes through them: A, then P, then E.
Questions and answers
What is the difference between a codon and an anticodon?
A codon is three bases on the mRNA. An anticodon is the matching three bases on a tRNA. They pair up, so each tRNA brings the amino acid its codon calls for.
What happens at the stop codon?
No tRNA matches a stop codon. A release factor enters the A site instead, the finished polypeptide is let go, and the ribosome comes apart from the mRNA.
Where does translation happen?
At ribosomes in the cytoplasm, free or on the rough endoplasmic reticulum. The mRNA is made in the nucleus by transcription and then carried out to them.