How it works
The parts of a bacteriophage, shown on T4, a virus that infects E. coli. The top figure is the phage on its own; the bottom figure shows it on a bacterium, tail contracted, passing its DNA into the cell.
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How the parts work together
The long tail fibres find receptors on the bacterium and signal the baseplate. The short fibres unfold and grip the cell, the sheath contracts, the tail tube punctures the outer membrane, and the DNA passes from the head down the tube.
How the head is filled
The head is built empty around a scaffolding protein, which is then broken down. A motor at a tiny pore packs the DNA in at up to 2,000 base pairs a second.
- Sheath and tube are easy to mix up: the sheath is the outer tube that contracts, the tube is inside it and carries the DNA.
Questions and answers
How big is a T4 bacteriophage?
About 90 nanometres wide and 200 nanometres long, which makes it a relatively large virus.
What is the head of a bacteriophage called?
The capsid. On T4 it is icosahedral and holds a double-stranded DNA genome of about 169,000 base pairs.
What do the tail fibres of a bacteriophage do?
The long tail fibres recognise receptors on the surface of the bacterium, so they decide which bacteria the phage can infect. The short tail fibres then bind the cell for good.