How it works
Label the essential steps of fermentation on two charts. The first follows glucose through glycolysis to lactate. The second starts at pyruvate and ends at ethanol and carbon dioxide.
Read the full guide
Lactic acid fermentation
Glycolysis splits glucose into two pyruvate and gains two ATP. It also turns NAD⁺ into NADH. Lactate dehydrogenase then passes the electrons of NADH to pyruvate, which becomes lactate, and NAD⁺ is made again for glycolysis. Muscle cells do this.
Alcoholic fermentation
Yeast starts the same way. Pyruvate decarboxylase takes carbon dioxide off pyruvate and leaves acetaldehyde. Alcohol dehydrogenase turns acetaldehyde into ethanol, and here too NADH becomes NAD⁺. The dashed arrows lead from glycolysis on the first chart and back to it.
- Rounded boxes are molecules, the square-cornered box is the stage, and boxes with cut corners are enzymes.
- The dots beside a molecule count its carbons.
Questions and answers
What are the steps of fermentation?
Glycolysis comes first: glucose is split into two pyruvate, with two ATP and two NADH made. Then pyruvate is turned into lactate, or into ethanol and carbon dioxide, and NADH is turned back into NAD⁺.
What is the difference between lactic acid and alcoholic fermentation?
In lactic acid fermentation pyruvate is turned straight into lactate. In alcoholic fermentation pyruvate first loses carbon dioxide and becomes acetaldehyde, which is then turned into ethanol.
What is the purpose of fermentation?
To make NAD⁺ again. Glycolysis needs NAD⁺ to keep going, and fermentation gets it back by passing the electrons of NADH to pyruvate or to acetaldehyde.
How much ATP does fermentation make?
By these two paths, two ATP for each glucose, all of it from glycolysis. The steps after pyruvate make no ATP.