• 27 blanks
  • Checked October 2026

Glycolysis Labeling Quiz

Choose what belongs in the lit blank, from glucose down to pyruvate, starting with the molecules.

Glycolysis Labeling Quiz

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What belongs in this blank?

1 of 11

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27
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Write the name of each numbered blank.Answer key

  1. Glucose
  2. Glucose 6-phosphate
  3. Fructose 6-phosphate
  4. Fructose 1,6-bisphosphate
  5. Dihydroxyacetone phosphate
  6. Glyceraldehyde 3-phosphate
  7. 1,3-Bisphosphoglycerate
  8. 3-Phosphoglycerate
  9. 2-Phosphoglycerate
  10. Phosphoenolpyruvate
  11. Pyruvate
  12. First ATP used
  13. Second ATP used
  14. NADH made
  15. First ATP made
  16. Water lost
  17. Second ATP made
  18. Hexokinase
  19. Phosphoglucose isomerase
  20. Phosphofructokinase
  21. Aldolase
  22. Triose phosphate isomerase
  23. Glyceraldehyde 3-phosphate dehydrogenase
  24. Phosphoglycerate kinase
  25. Phosphoglycerate mutase
  26. Enolase
  27. Pyruvate kinase

Drawn for this site on 2026-10-01 by script from plain geometry. Licence: own work.

Each numbered ring is a button. Use the arrow keys to move between them and Enter to choose. Names are read out in Study, and once a blank is answered.

Next step Next: the citric acid cycleGlycolysis as one stage of cellular respiration

All 27 blanks on the diagram

Show the list

Molecules: 11 blanks 11

  1. Glucose Glucose is the sugar that enters glycolysis. One glucose leaves the pathway as two pyruvate.
  2. Glucose 6-phosphate Glucose 6-phosphate is glucose with one phosphate added. Hexokinase makes it, and the step uses one ATP.
  3. Fructose 6-phosphate Fructose 6-phosphate is an isomer of glucose 6-phosphate. Step 2 rearranges an aldose into a ketose.
  4. Fructose 1,6-bisphosphate Fructose 1,6-bisphosphate carries two phosphate groups. Aldolase splits it: two three-carbon sugar phosphates.
  5. Dihydroxyacetone phosphate Dihydroxyacetone phosphate is one half of the split sugar. Triose phosphate isomerase converts it to glyceraldehyde 3-phosphate.
  6. Glyceraldehyde 3-phosphate Glyceraldehyde 3-phosphate is the half that goes on. Each glucose gives two of it, so steps 6 to 10 run twice.
  7. 1,3-Bisphosphoglycerate 1,3-Bisphosphoglycerate forms as glyceraldehyde 3-phosphate is oxidized. It gives one phosphate to ADP in the next step.
  8. 3-Phosphoglycerate 3-Phosphoglycerate is left when one phosphate goes to ADP. Its phosphate sits on carbon 3.
  9. 2-Phosphoglycerate 2-Phosphoglycerate has its phosphate on carbon 2. Phosphoglycerate mutase makes it from 3-phosphoglycerate.
  10. Phosphoenolpyruvate Phosphoenolpyruvate forms when enolase removes water. It passes its phosphate to ADP in step 10.
  11. Pyruvate Pyruvate is the end product of glycolysis. One glucose gives two pyruvate.

In and out: 6 blanks 6

  1. First ATP used Step 1 uses one ATP. Its phosphate group goes onto glucose.
  2. Second ATP used Step 3 uses the second ATP. Its phosphate goes onto fructose 6-phosphate.
  3. NADH made Step 6 makes NADH from NAD⁺. No other step of glycolysis does. One glucose gives two NADH.
  4. First ATP made Step 7 makes the first ATP. A phosphate moves to ADP from 1,3-bisphosphoglycerate.
  5. Water lost Step 9 gives off one water molecule. Enolase removes it from 2-phosphoglycerate.
  6. Second ATP made Step 10 makes the second ATP. A phosphate moves from phosphoenolpyruvate to ADP.

Enzymes: 10 blanks 10

  1. Hexokinase Hexokinase adds a phosphate from ATP to glucose. This is step 1.
  2. Phosphoglucose isomerase Phosphoglucose isomerase rearranges glucose 6-phosphate into fructose 6-phosphate. This is step 2.
  3. Phosphofructokinase Phosphofructokinase adds a second phosphate from ATP. Step 3 limits the rate of glycolysis.
  4. Aldolase Aldolase splits fructose 1,6-bisphosphate into two three-carbon molecules. This is step 4.
  5. Triose phosphate isomerase Triose phosphate isomerase converts dihydroxyacetone phosphate to glyceraldehyde 3-phosphate. This is step 5.
  6. Glyceraldehyde 3-phosphate dehydrogenase This enzyme oxidizes glyceraldehyde 3-phosphate and makes NADH. It is the enzyme of step 6.
  7. Phosphoglycerate kinase Phosphoglycerate kinase moves a phosphate to ADP from 1,3-bisphosphoglycerate. Step 7 makes ATP.
  8. Phosphoglycerate mutase Phosphoglycerate mutase moves the phosphate from carbon 3 to carbon 2. This is step 8.
  9. Enolase Enolase removes water from 2-phosphoglycerate. Step 9 makes phosphoenolpyruvate.
  10. Pyruvate kinase Pyruvate kinase moves a phosphate from phosphoenolpyruvate to ADP. Step 10 makes pyruvate and ATP.

How this list was compiled: Every name, the order of the ten steps and what each step uses or makes were checked on 2026-10-01 in two texts, read for facts only with nothing copied: Wikipedia, Glycolysis (https://en.wikipedia.org/wiki/Glycolysis), and the page Glycolysis and Fermentation of a university course in molecular biochemistry, read in its archived copy (https://web.archive.org/web/2015/https://www.rpi.edu/dept/bcbp/molbiochem/MBWeb/mb1/part2/glycolysis.htm). The layout of the chart, the hints and the detail lines are our own. Licence: own work

The drawing: Drawn for this site on 2026-10-01 by script from plain geometry. Own original chart: the layout is ours and nothing was traced or copied. Licence: own work

How it works

This glycolysis labeling quiz draws the pathway as a chart of numbered blanks with no names. It opens on the molecules from glucose to pyruvate. The enzymes are a second set, and the ATP, NADH and water a third.

Read the full guide

Name the lit blank

A blank is lit when the page opens. Pick from the four choices. They all come from one set, so an enzyme is never offered for a molecule. The first round is the molecules, the next what goes in and comes out, then the enzymes.

Read the chart

The chart has two views, steps 1 to 5 and steps 6 to 10, and the page shows the one the question is on. An arrow that points into the pathway means something is used. An arrow that points away means something is made.

  • ATP goes in at steps 1 and 3 and comes out at steps 7 and 10.
  • The four kinases sit at the four steps where ATP is used or made.
  • Steps 6 to 10 run twice for each glucose, because step 4 splits one sugar into two halves.

Questions and answers

What are the 10 steps of glycolysis in order?

By enzyme: hexokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, triose phosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase, enolase and pyruvate kinase. Together they turn one glucose into two pyruvate.

Where does glycolysis take place?

In the cytosol, the liquid part of the cell. All ten steps happen there.

How many ATP does glycolysis make?

Four ATP are made and two are used, so the net gain is two ATP for each glucose. The pathway also makes two NADH and ends with two pyruvate.

Which steps of glycolysis use ATP?

Steps 1 and 3. Hexokinase uses one ATP to add a phosphate to glucose, and phosphofructokinase uses a second to make fructose 1,6-bisphosphate. Steps 7 and 10 make ATP.

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