How it works
On this lac operon quiz diagram a gene, a control site or a protein is named and you tap it on the chart. Eight parts are asked with no lactose, then six with it. What you miss is offered again after each chart.
Read the full guide
Pairs that get mixed up
The promoter is the box under RNA polymerase; the operator is the short box after it, under the repressor. A gene is a box on the DNA and its protein is a shape under the mRNA, so lacZ and beta-galactosidase are two answers. On the second chart the protein that has left the DNA, with allolactose in its top, is the inactive repressor.
Read the DNA left to right
The regulatory gene lacI stands alone at the left end. Then come the promoter, the operator and the structural genes in the order Z, Y, A, with lacZ drawn longest. The three proteins stand under their genes in the same order.
- The I of lacI stands for inducibility.
Questions and answers
Is the lac operon inducible or repressible?
Inducible. Its genes stay off until an inducer, allolactose, takes the repressor off the operator. Because the switch is a repressor, the control is called negative inducible.
Is lacI part of the lac operon?
No. lacI lies beside the operon with a promoter of its own and is expressed all the time, so the cell always holds some repressor.
What does polycistronic mean for the lac operon?
One transcript that codes for several proteins. lacZ, lacY and lacA share one promoter and are copied into a single mRNA, so the three are switched on and off together.