How it works
When a tube of blood is spun in a centrifuge, it separates into three layers. The heaviest part, the red blood cells, sinks to the bottom, the plasma stays on top, and a thin buffy coat lies between them.
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The three layers
Plasma is the top layer, about 55 percent of the blood. The buffy coat is the thin band under it. The erythrocytes are packed at the bottom.
What each layer holds
Follow the lines from a layer to its tiles. Plasma is mostly water, with plasma proteins and other solutes. The proteins come in three groups: albumins, globulins and fibrinogen. The buffy coat holds the leukocytes and the platelets.
- The drawing is not to scale: the real buffy coat is far thinner than drawn here.
Questions and answers
What are the components of whole blood after centrifugation?
Three layers. Plasma, the liquid, is on top. The buffy coat, a thin band of white blood cells and platelets, is in the middle. The erythrocytes, or red blood cells, are packed at the bottom.
What is the buffy coat?
The thin layer between the plasma and the red cells in a tube of spun blood. It holds most of the white blood cells and the platelets.
What is plasma made of?
Mostly water. Dissolved in it are the plasma proteins (albumins, globulins and fibrinogen) and other solutes such as salts, nutrients, gases, hormones and wastes.
What does hematocrit mean?
It is the share of the blood that is made up of red blood cells: the bottom layer of the tube compared with the whole column.
How do I drag the labels to identify the components of whole blood after centrifugation?
Name the three layers first, top to bottom: plasma, buffy coat, erythrocytes. Then place what each one holds. This drawing is our own, made for practice.