Digestive System Worksheet

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  1. 1.Which order does food travel in?
  2. 2.Where does food go straight after the esophagus?
  3. 3.What are the three parts of the small intestine, in order?
  4. 4.Which ring of muscle lets chyme out of the stomach into the duodenum?
  5. 5.Which valve separates the small intestine from the large intestine?
  6. 6.Which part of the large intestine comes first?
  7. 7.After the transverse colon, where does waste go next?
  8. 8.What moves food along the esophagus and intestines?
  9. 9.Which part of the digestive tract is longest?
  10. 10.About how long is the large intestine?
  11. 11.About how long is the esophagus in an adult?
  12. 12.Which is the small intestine's shortest part?
  13. 13.Where is most food digested and absorbed?
  14. 14.What does the large intestine mainly absorb?
  15. 15.Which organ makes bile?
  16. 16.Which organ stores and concentrates bile?
  17. 17.Which organ sends juice with bicarbonate and enzymes into the duodenum?
  18. 18.Which part of the small intestine absorbs vitamin B12 and bile salts?
  19. 19.Which part of the small intestine receives bile and pancreatic juice?
  20. 20.What do villi do?
  21. 21.Which of these is an accessory organ, not part of the tube food passes through?
  22. 22.Which is the human body's largest gland?
  23. 23.Which cells of the stomach lining make hydrochloric acid?
  24. 24.The pancreas also makes a hormone that lowers blood sugar. Which one?
  25. 25.Which enzyme in saliva starts breaking down starch?
  26. 26.Which enzyme starts protein digestion in the stomach?
  27. 27.Which enzyme breaks down fats?
  28. 28.Which protein-cutting enzyme is made by the pancreas and works in the small intestine?
  29. 29.What does bile do to fats?
  30. 30.What is the pH of stomach acid?
  31. 31.Which two jobs does stomach acid do?
  32. 32.Why does the pancreas add bicarbonate to the duodenum?
  33. 33.Proteins are finally broken down into…
  34. 34.Which is mechanical digestion, not chemical?
  35. 35.Where does chemical digestion of starch begin?
  36. 36.Salivary amylase breaks starch down into which sugars?
  37. 37.Chyme leaving the stomach is…
About how long is the large intestine?

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Unofficial practice questions, not affiliated with any school, textbook publisher or curriculum. Bank checked September 24, 2026.

How it works

This digestive system worksheet has 37 questions: the path food takes from mouth to rectum, what each organ does, and chemical digestion with amylase, pepsin, lipase, trypsin, stomach acid and bile.

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Three sets

The path of food covers the order of the organs, the sphincters and valves, peristalsis and lengths. Organs and their jobs covers the liver, gallbladder, pancreas and intestines. Enzymes and chemical digestion covers what breaks down what, and where.

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  • Amylase for starch, pepsin and trypsin for protein, lipase for fat.
  • The liver makes bile; the gallbladder only stores it and squeezes it into the duodenum after a meal.
  • Small intestine: most absorption. Large intestine: water and salts.

Questions and answers

What is the order of the digestive system?

Mouth, pharynx, esophagus, stomach, small intestine (duodenum, jejunum, ileum), large intestine (cecum, colon, rectum) and anus. The liver, gallbladder and pancreas add juices but food does not pass through them.

Where does most digestion happen?

In the small intestine, about 6.5 m long. Bile and pancreatic juice enter the duodenum, enzymes finish breaking food down, and villi lining the jejunum and ileum absorb most nutrients into the blood.

What is the difference between mechanical and chemical digestion?

Mechanical digestion breaks food into smaller pieces, by chewing and by the stomach churning. Chemical digestion uses enzymes and acid to break the molecules themselves apart.

All 37 questions with answers

The path of food 12

  • About how long is the esophagus in an adult? Answer: 25 cm (10 in). Source: Wikipedia, Esophagus. Read 2026-09-24.
  • About how long is the large intestine? Answer: 1.5 m (5 ft). Source: Wikipedia, Large intestine. Read 2026-09-24.
  • After the transverse colon, where does waste go next? Answer: Down the descending colon to the rectum. Source: Wikipedia, Large intestine. Read 2026-09-24.
  • What are the three parts of the small intestine, in order? Answer: Duodenum, jejunum, ileum. Source: Wikipedia, Small intestine. Read 2026-09-24.
  • What moves food along the esophagus and intestines? Answer: Peristalsis: waves of muscle contraction. Source: Wikipedia, Human digestive system. Read 2026-09-24.
  • Where does food go straight after the esophagus? Answer: The stomach. Source: Wikipedia, Esophagus. Read 2026-09-24.
  • Which is the small intestine's shortest part? Answer: The duodenum. Source: Wikipedia, Duodenum. Read 2026-09-24.
  • Which order does food travel in? Answer: Mouth, esophagus, stomach, small intestine, large intestine. Source: Wikipedia, Human digestive system. Read 2026-09-24.
  • Which part of the digestive tract is longest? Answer: The small intestine. Source: Wikipedia, Small intestine. Read 2026-09-24.
  • Which part of the large intestine comes first? Answer: The cecum. Source: Wikipedia, Large intestine. Read 2026-09-24.
  • Which ring of muscle lets chyme out of the stomach into the duodenum? Answer: The pyloric sphincter. Source: Wikipedia, Stomach. Read 2026-09-24.
  • Which valve separates the small intestine from the large intestine? Answer: The ileocecal valve. Source: Wikipedia, Ileum. Read 2026-09-24.
Show all 37 questions (25 more)

Organs and their jobs 12

  • The pancreas also makes a hormone that lowers blood sugar. Which one? Answer: Insulin. Source: Wikipedia, Pancreas. Read 2026-09-24.
  • What do villi do? Answer: Give the small intestine a huge surface for absorption. Source: Wikipedia, Small intestine. Read 2026-09-24.
  • What does the large intestine mainly absorb? Answer: Water and salts. Source: Wikipedia, Large intestine. Read 2026-09-24.
  • Where is most food digested and absorbed? Answer: The small intestine. Source: Wikipedia, Small intestine. Read 2026-09-24.
  • Which cells of the stomach lining make hydrochloric acid? Answer: Parietal cells. Source: Wikipedia, Gastric acid. Read 2026-09-24.
  • Which is the human body's largest gland? Answer: The liver. Source: Wikipedia, Liver. Read 2026-09-24.
  • Which of these is an accessory organ, not part of the tube food passes through? Answer: The liver. Source: Wikipedia, Human digestive system. Read 2026-09-24.
  • Which organ makes bile? Answer: The liver. Source: Wikipedia, Bile. Read 2026-09-24.
  • Which organ sends juice with bicarbonate and enzymes into the duodenum? Answer: The pancreas. Source: Wikipedia, Pancreas. Read 2026-09-24.
  • Which organ stores and concentrates bile? Answer: The gallbladder. Source: Wikipedia, Bile. Read 2026-09-24.
  • Which part of the small intestine absorbs vitamin B12 and bile salts? Answer: The ileum. Source: Wikipedia, Ileum. Read 2026-09-24.
  • Which part of the small intestine receives bile and pancreatic juice? Answer: The duodenum. Source: Wikipedia, Duodenum. Read 2026-09-24.

Enzymes and chemical digestion 13

  • Chyme leaving the stomach is… Answer: Partly digested food mixed with gastric juice. Source: Wikipedia, Stomach. Read 2026-09-24.
  • Proteins are finally broken down into… Answer: Amino acids. Source: Wikipedia, Trypsin. Read 2026-09-24.
  • Salivary amylase breaks starch down into which sugars? Answer: Maltose and glucose. Source: Wikipedia, Salivary gland. Read 2026-09-24.
  • What does bile do to fats? Answer: Breaks them into small droplets (emulsifies them). Source: Wikipedia, Bile. Read 2026-09-24.
  • What is the pH of stomach acid? Answer: About 1 to 3. Source: Wikipedia, Gastric acid. Read 2026-09-24.
  • Where does chemical digestion of starch begin? Answer: In the mouth. Source: Wikipedia, Human digestive system. Read 2026-09-24.
  • Which enzyme breaks down fats? Answer: Lipase. Source: Wikipedia, Pancreas. Read 2026-09-24.
  • Which enzyme in saliva starts breaking down starch? Answer: Amylase. Source: Wikipedia, Salivary gland. Read 2026-09-24.
  • Which enzyme starts protein digestion in the stomach? Answer: Pepsin. Source: Wikipedia, Gastric acid. Read 2026-09-24.
  • Which is mechanical digestion, not chemical? Answer: Chewing and the stomach churning food. Source: Wikipedia, Human digestive system. Read 2026-09-24.
  • Which protein-cutting enzyme is made by the pancreas and works in the small intestine? Answer: Trypsin. Source: Wikipedia, Trypsin. Read 2026-09-24.
  • Which two jobs does stomach acid do? Answer: Kills germs and activates pepsin. Source: Wikipedia, Gastric acid. Read 2026-09-24.
  • Why does the pancreas add bicarbonate to the duodenum? Answer: To neutralise the acid coming from the stomach. Source: Wikipedia, Pancreas. Read 2026-09-24.

How this list was compiled: Questions, wrong choices and explanations were written for this site. Facts were checked on Wikipedia (Human digestive system, Esophagus, Stomach, Gastric acid, Small intestine, Duodenum, Ileum, Large intestine, Bile, Liver, Pancreas, Trypsin, Salivary gland), each named in the question's source line and read 2026-09-24. Licence: own work