How it works
Each drawing is a slice through the edge of a bacterium, with the outside at the top and the plasma membrane near the bottom. All three walls hold peptidoglycan. What differs is how much, and what lies outside it.
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Thick wall or two membranes
A Gram-positive wall is mostly peptidoglycan, with teichoic acids threaded through it. A Gram-negative wall has only a thin layer, and outside it a second membrane that carries porins and lipopolysaccharide.
The acid-fast wall
Here the thin peptidoglycan is linked to arabinogalactan, and that to a tall layer of mycolic acids. Mycolic acids are long fatty acids, so the outside of this wall is waxy.
- A teichoic acid stops in the peptidoglycan; a lipoteichoic acid reaches down into the membrane.
Questions and answers
What is the difference between a Gram-positive and a Gram-negative cell wall?
A Gram-positive wall has a thick layer of peptidoglycan with teichoic acids and no outer membrane. A Gram-negative wall has a thin layer of peptidoglycan lying between two membranes, and its outer membrane carries lipopolysaccharide.
Where are endotoxins on a bacterial cell diagram?
In the Gram-negative wall's outer membrane. Endotoxin is another name for lipopolysaccharide, and more exactly for lipid A, the part that anchors each chain in the membrane.
What is an acid-fast cell wall made of?
From the inside out: a thin layer of peptidoglycan, a layer of arabinogalactan linked to it, and an outer layer of mycolic acids. The mycolic acids make up most of the wall.
Why are the walls called Gram-positive and Gram-negative?
The names come from the Gram stain. A thick layer of peptidoglycan holds the stain, so the cell tests positive. A thin layer does not hold it, so the cell tests negative.