How it works
This blank bacterial cell wall diagram stacks three slices: Gram-positive at the top, Gram-negative in the middle, acid-fast at the bottom. In each, the outside of the cell is up. Fourteen names go on.
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The fourteen names, wall by wall
Gram-positive: peptidoglycan, teichoic acid, lipoteichoic acid, plasma membrane. Gram-negative: outer membrane, lipopolysaccharide, porin, peptidoglycan, periplasm, plasma membrane. Acid-fast: mycolic acids, arabinogalactan, peptidoglycan, plasma membrane.
Which pattern takes which name
Brickwork is peptidoglycan: a thick slab at the top, a thin strip in the other two. A double row of round heads is a membrane: the lowest in each drawing is the plasma membrane, and the upper one of the middle drawing the outer membrane. A wavy chain that ends in the brickwork is a teichoic acid; one that goes down to the membrane is a lipoteichoic acid. Bead strings are lipopolysaccharide, barrels porins, and the clear space between the two membranes periplasm. Forked lines are arabinogalactan and the tall upright lines mycolic acids.
- The speckled strip under each plasma membrane is cytoplasm. It takes no name.
- The sheet has no label lines. Each name goes on its own layer, or at the end of a line you rule to the margin.
- Murein is another name for peptidoglycan, and cytoplasmic membrane for the plasma membrane. A course may use either.
Questions and answers
What color do Gram-positive and Gram-negative bacteria stain?
Gram-positive cells keep the crystal violet stain and look purple under the microscope. Gram-negative cells lose it at the decolorizing step, take up the counterstain and look red or pink.
What is peptidoglycan made of?
Sugars and amino acids. Chains of two alternating sugars, N-acetylglucosamine and N-acetylmuramic acid, are cross-linked through short peptides of three to five amino acids into a mesh. The brickwork on the sheet stands for that mesh.
What does acid-fast mean?
That the stained cell resists decolorization with an acid-alcohol mixture: it keeps the first dye, carbol fuchsin, and appears red. A wall with a waxy outer layer rich in mycolic acids resists acids in this way.
Do all bacteria have peptidoglycan in the cell wall?
Nearly all. The mycoplasmas are an exception: they lack the peptidoglycan layer. Where there is one, it lies immediately outside the cell membrane.